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- Title
Mitochondrial fragmentation caused by phenanthroline promotes mitophagy
- Authors
Park, So Jung; Shin, Ji Hyun; Kim, Eun Sung; Jo, Yoon Kyung; Kim, Jung Ho; Hwang, Jung Jin; Kim, Jin Cheon; Cho, Dong-Hyung
- Abstract
Abstract: Mitochondrial dynamics and mitophagy are thought to be important events for the quality control of mitochondria and mitochondria-associated diseases. To identify novel mitophagy modulators, we developed a cell-based screening system and selected 1,10-phenanthroline (Phen) as a target molecule. Phen treatment highly induced mitochondrial fragmentation and mitochondrial dysfunctions in a Drp1 dependent manner. Phen treatment also increased autophagy. Moreover, prolonged exposure of Phen increased mitochondria clearance through mitophagy. Phen-mediated loss of mitochondrial mass was more reduced in ATG5 deficient cells than in wild type cells. In addition, down-regulation of Drp1 decreased autophagy activation, suggesting that mitochondrial fission is involved in Phen-mediated mitophagy. Thus, our results demonstrate that the disruption of mitochondrial dynamics and mitochondrial dysfunctions provokes mitophagy in Phen-treated cells.
- Subjects
MITOCHONDRIA; PHENANTHROLINE; NEURODEGENERATION; AUTOPHAGY; MITOCHONDRIAL pathology; CYTOLOGICAL techniques
- Publication
FEBS Letters, 2012, Vol 586, Issue 24, p4303
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.10.035