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- Title
MiR-34a is involved in Tat-induced HIV-1 long terminal repeat (LTR) transactivation through the SIRT1/NFκB pathway
- Authors
Zhang, Hong-Sheng; Chen, Xin-Yu; Wu, Tong-Chao; Sang, Wei-Wei; Ruan, Zheng
- Abstract
Abstract: MicroRNAs (miRNAs) regulate gene expression and may contribute to HIV-1 infection. In this study, our goal was to investigate the mechanisms by which miR-34a influenced Tat-induced HIV-1 transactivation through the SIRT1/NFκB pathway. We showed that Tat induced up-regulation of miR-34a expression in TZM-bl cells. MiR-34a significantly inhibited SIRT1 expression. Overexpression of miR-34a increased Tat-induced LTR transactivation. Forced expression of miR-34a decreased SIRT1 protein expression and consequently diminished Tat-induced acetylation of p65, while treatment with a miR-34a inhibitor had the opposite effect. These results suggest that regulating SIRT1 by down-regulation of miR-34a levels may be a therapeutic strategy against HIV-1 replication.
- Subjects
MICRORNA; GENE expression; HIV infections; ACETYLATION; GENETIC regulation; NF-kappa B
- Publication
FEBS Letters, 2012, Vol 586, Issue 23, p4203
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.10.023