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- Title
Distinctive heat-shock response of bioleaching microorganism Acidithiobacillus ferrooxidans observed using genome-wide microarray.
- Authors
Yin, Huaqun; Tang, Min; Zhou, Zhijun; Fu, Xian; Shen, Li; Liang, Yili; Li, Qian; Liu, Hongwei; Liu, Xueduan
- Abstract
Temperature plays an important role in the heap bioleaching. The maldistribution of ventilation in the heap leads to local hyperthermia, which does exert a tremendous stress on bioleaching microbes. In this study, the genome-wide expression profiles of Acidithiobacillus ferrooxidans at 40 °C were detected using the microarray. The results showed that some classic proteases like Lon and small heat-shock proteins were not induced, and heat-inducible membrane proteins were suggested to be under the control of σE. Moreover, expression changes of energy metabolism are noteworthy, which is different from that in heterotrophic bacteria upon heat stress. The induced enzymes catalyzed the central carbon metabolism pathway that might mainly provide precursors of amino acids for protein synthesis. These results will deepen the understanding of the mechanisms of heat-shock response on autotrophic bacteria.
- Subjects
BACTERIA heat shock proteins; BACTERIAL leaching; THIOBACILLUS ferrooxidans; GENOMES; PROTEIN microarrays; GENE expression; PROTEOLYTIC enzymes
- Publication
Canadian Journal of Microbiology, 2012, Vol 58, Issue 5, p628
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/w2012-023