We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Impairment of Gingival Fibroblast Adherence by IL-6/SIL-6R.
- Authors
Naruishi, K.; Takashiba, S.; Nishimura, F.; Chou, H.-H.; Arai, H.; Yamada, H.; Murayama, Y.
- Abstract
Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gpl3O and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBPrP. Cytoskeletal β-actin and adhesion molecule integrin-α5, a subunit of α5β1 integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of β-actin and integrin-α5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.
- Subjects
FIBROBLAST adhesion; INTERLEUKIN-6; INTEGRINS; TRANSCRIPTION factors; ACTIN; CELL adhesion -- Molecular aspects; BINDING sites; BIOCHEMISTRY technique
- Publication
Journal of Dental Research, 2001, Vol 80, Issue 5, p1421
- ISSN
0022-0345
- Publication type
Article
- DOI
10.1177/00220345010800050701