We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Sodium glucose-linked transport in the ruminal epithelium of fallow deer – comparison to sheep.
- Authors
Aschenbach, J. R.; Borau, T.; Gäbel, G.
- Abstract
A higher expression of the sodium glucose-linked transporter, SGLT-1, has been previously demonstrated in the intestine of adult fallow deer compared to sheep. The functional presence of SGLT-1 in the rumen of both species was investigated in the present study. Isolated ruminal epithelia were incubated in Ussing chambers. D-Glucose or 3-O-methyl-α-D-glucose (3-OMG) were applied at 10 mmol·l–1 to the mucosal side of tissues and the changes in short-circuit current (ΔIsc) were recorded. Alternatively, apical uptake of [14C]-D-glucose (200 µmol·l–1) was determined in the presence or absence of phlorizin (100 µmol·l–1) or Na+ (115 mmol·l–1). In both species, mucosal D-glucose addition induced a larger (P<0.05) ΔIsc than the mucosal addition of 3-OMG. When comparing the effects of D-glucose or 3-OMG between species, the ΔIsc was two-fold larger (P<0.01) in sheep compared to fallow deer. Accordingly, phlorizin-sensitive D-glucose uptake was ~50% smaller (P<0.05) in fallow deer. It is concluded that D-glucose can be absorbed via SGLT-1 from the forestomachs of both species. However, the intermediate-mixed feeding habit of fallow deer does not seem to coincide with a higher activity of the ruminal SGLT-1.
- Subjects
FALLOW deer; SHEEP; INTESTINES; GLUCOSE; SODIUM; EPITHELIUM
- Publication
Journal of Comparative Physiology B: Biochemical, Systemic & Environmental Physiology, 2002, Vol 172, Issue 7, p561
- ISSN
0174-1578
- Publication type
Article
- DOI
10.1007/s00360-002-0283-y