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- Title
Isolation of a simian immunodeficiency virus from a malbrouck ( Chlorocebus cynosuros).
- Authors
Carr, Michael; Kawaguchi, Akira; Sasaki, Michihito; Gonzalez, Gabriel; Ito, Kimihito; Thomas, Yuka; Hang'ombe, Bernard; Mweene, Aaron; Zhao, Guoyan; Wang, David; Orba, Yasuko; Ishii, Akihiro; Sawa, Hirofumi
- Abstract
To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4 T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck ( Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.
- Subjects
SIMIAN immunodeficiency virus; CD4 antigen; T cells; LYMPHOCYTES; POLYMERASE chain reaction
- Publication
Archives of Virology, 2017, Vol 162, Issue 2, p543
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-016-3129-8