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- Title
Assessing the Risks of Genotoxicity in the Therapeutic Development of Induced Pluripotent Stem Cells.
- Authors
Hong, So Gun; Dunbar, Cynthia E; Winkler, Thomas
- Abstract
Induced pluripotent stem cells (iPSCs) have great potential for regenerative medicine as well as for basic and translational research. However, following the initial excitement over the enormous prospects of this technology, several reports uncovered serious concerns regarding its safety for clinical applications and reproducibility for laboratory applications such as disease modeling or drug screening. In particular, the genomic integrity of iPSCs is the focus of extensive research. Epigenetic remodeling, aberrant expression of reprogramming factors, clonal selection, and prolonged in vitro culture are potential pathways for acquiring genomic alterations. In this review, we will critically discuss current reprogramming technologies particularly in the context of genotoxicity, and the consequences of these alternations for the potential applications of reprogrammed cells. In addition, current strategies of genetic modification of iPSCs, as well as applicable suicide strategies to control the risk of iPSC-based therapies will be introduced.
- Subjects
GENETIC toxicology; PLURIPOTENT stem cells; REGENERATIVE medicine; GENE therapy; EPIGENETICS
- Publication
Molecular Therapy, 2013, Vol 21, Issue 2, p272
- ISSN
1525-0016
- Publication type
Article
- DOI
10.1038/mt.2012.255