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- Title
Experimental Peritoneal Metastasis Model: Which Type of Rodents Should we Choose, and which Method Should we Perform for the Intraperitoneal Inoculation of Tumor Cells?
- Authors
Manoğlu, Berke; Bişgin, Tayfun; Canda, Aras Emre; Aktaş, Safiye; Altun, Zekiye Sultan; Yılmaz, Osman
- Abstract
Aim: This study aimed to create a peritoneal metastasis (PM) model in Wistar albino rats and nude mice and compare PM models and different tumor cell inoculation methods in the two experimental animal types. Method: There were two main groups: group 1 comprised Wistar albino rats (n=16), and group 2 comprised nude mice (n=16). The group comprising rats was divided into two subgroups (1A and 1B), to which different tumor inoculation methods were applied. Group 2, comprising nude mice, was divided into two subgroups (2A and 2B), to which different tumor inoculation methods were applied. Euthanization was performed on the 7th and 14th days after tumor inoculation. The obtained samples were evaluated macroscopically, microscopically, and biochemically. Results: Although no PM model was formed in group 1, a PM model occurred in the subjects in group 2 who were euthanized on the 14th day. There was no statistically significant difference between the mean peritoneal carcinomatosis index scores, tumor diameters, and the amount of intraabdominal ascites in the subgroups (2A vs. 2B), in which the PM model was created by two different methods. Conclusion: The inoculation of tumor cells with the peritoneal injection method enabled the creation of a PM model that can be used in experimental studies. Although a PM model could not be established in rats, a complete PM model was established in nude mice. In future studies, we plan to evaluate the efficacies of different drugs in the PM models we have created.
- Subjects
THERAPEUTIC use of antineoplastic agents; RODENTS; BIOLOGICAL models; ANIMAL experimentation; METASTASIS; INTRAPERITONEAL injections; PERITONEUM tumors; COLORECTAL cancer; RATS; TREATMENT effectiveness; DESCRIPTIVE statistics; RESEARCH funding; DECISION making in clinical medicine; MICE
- Publication
Turkish Journal of Colorectal Disease, 2023, Vol 33, Issue 3, p80
- ISSN
2536-4898
- Publication type
Article
- DOI
10.4274/tjcd.galenos.2023.2023-5-5