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- Title
Molecular Analysis of Anti-Tuberculosis Drug Resistance of Mycobacterium tuberculosis Isolated in the Republic of Korea.
- Authors
Jeon, Se-Mi; Park, Sanghee; Lim, Na-Ra; Lee, Noori; Jung, Jihee; Sung, Nackmoon; Kim, Seonghan
- Abstract
Rapid and accurate detection of tuberculosis (TB) drug resistance is critical for the successful treatment and control of TB. Here, we investigated resistance to anti-TB drugs and genetic variations in 215 drug-resistant Mycobacterium tuberculosis isolates in Korea. Genetic variations were observed in rpoB Ser531Leu, katG Ser315Thr, and gyrA Asp94Gly; however, the minimum inhibitory concentrations varied, which can be attributed to other resistance mechanisms. Examination of genetic relatedness among drug-resistant isolates revealed that the cluster size of resistant bacteria was less than six strains, suggesting no evidence of a large-scale epidemic caused by a specific strain. However, rpoC mutants of the rifampicin-resistant isolates were composed of five types of clusters, suggesting that these compensatory mutations advance propagation. In the present study, more than 90% of the resistance mechanisms to major anti-TB drugs were identified, and the effect of each mutation on drug resistance was estimated. With the clinical application of recent next-generation sequencing-based susceptibility testing, the present study is expected to improve the clinical utilization of genotype-based drug susceptibility testing for the diagnosis and treatment of patients with drug-resistant TB.
- Subjects
SOUTH Korea; MYCOBACTERIUM tuberculosis; DRUG resistance; MYCOBACTERIA; ANTITUBERCULAR agents; DRUG analysis; DRUG utilization; TUBERCULOUS meningitis
- Publication
Antibiotics (2079-6382), 2023, Vol 12, Issue 8, p1324
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics12081324