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- Title
High-quality reference transcript datasets hold the key to transcript-specific RNA-sequencing analysis in plants.
- Authors
Brown, John W. S.; Calixto, Cristiane P. G.; Zhang, Runxuan
- Abstract
525I.525II.526III.527IV.527V.529VI.529529References529 Summary: Re‐programming of the transcriptome involves both transcription and alternative splicing (AS). Some genes are regulated only at the AS level with no change in expression at the gene level. AS data must be incorporated as an essential aspect of the regulation of gene expression. RNA‐sequencing (RNA‐seq) can deliver both transcriptional and AS information, but accurate methods to analyse the added complexity in RNA‐seq data are needed. The construction of a comprehensive reference transcript dataset (RTD) for a specific plant species, variety or accession, from all available sequence data, will immediately allow more robust analysis of RNA‐seq data. RTDs will continually evolve and improve, a process that will be more efficient if resources across a community are shared and pooled.
- Subjects
ARABIDOPSIS; GENE expression in plants; ALTERNATIVE RNA splicing; RNA sequencing; RNA analysis
- Publication
New Phytologist, 2017, Vol 213, Issue 2, p525
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.14208