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- Title
Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs.
- Authors
Domínguez, Juan; Acosta, Fermín; Pérez-Lago, Laura; Sambrano, Dilcia; Batista, Victoria; De La Guardia, Carolina; Abascal, Estefanía; Chiner-Oms, Álvaro; Comas, Iñaki; González, Prudencio; Bravo, Jaime; Del Cid, Pedro; Rosas, Samantha; Muñoz, Patricia; Goodridge, Amador; de Viedma, Darío García; García de Viedma, Darío
- Abstract
Systematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit-variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion (50%) of Mycobacterium tuberculosis isolates included in 6 clusters (A-F) in 2 provinces (Panama and Colon). Cluster A corresponded to the Beijing sublineage. Whole-genome sequencing (WGS) differentiated clusters due to active recent transmission, with low single-nucleotide polymorphism-based diversity (cluster C), from clusters involving long-term prevalent strains with higher diversity (clusters A, B). Prospective application in Panama of 3 tailored strain-specific PCRs targeting marker single-nucleotide polymorphisms identified from WGS data revealed that 31.4% of incident cases involved strains A-C and that the Beijing strain was highly represented and restricted mainly to Colon. Rational integration of MIRU-VNTR, WGS, and tailored strain-specific PCRs could be a new model for tuberculosis surveillance in countries without molecular/genomic epidemiology programs.
- Subjects
PANAMA; MOLECULAR epidemiology; MYCOBACTERIUM tuberculosis; TUBERCULOSIS; EPIDEMIOLOGY
- Publication
Emerging Infectious Diseases, 2019, Vol 25, Issue 3, p507
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2503.181593