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- Title
Hepatoprotective Effect of Kaempferol Against Alcoholic Liver Injury in Mice.
- Authors
Meng Wang; Jianguo Sun; Zhihui Jiang; Wenyan Xie; Xiaoying Zhang
- Abstract
Kaempferol is a biologically active component present in various plants. The hepatoprotective effect of kaempferol in drug-induced liver injury has been proven, while its effect against alcoholic liver injury (ALI) remains unclear. Hence, the present study aimed to evaluate the effect of kaempferol against ALI in mice. The experimental ALI mice model was developed and the mice were treated with different doses of kaempferol for 4 weeks. The liver functions were observed by monitoring the following parameters: Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) levels in serum; histopathological studies of liver tissue; oxidative stress by hydrogen peroxide (H2O2), superoxide dismutase (SOD) and glutathione (GSH); the lipid peroxidation status by malondialdehyde (MDA) and lipid accumulation by triglyceride (TG) level in serum; and the expression levels and activities of a key microsomal enzyme cytochrome 2E1 (CYP2E1), by both in vitro and in vivo methods. The ALI mice (untreated) showed clear symptoms of liver injury, such as significantly increased levels of oxidative stress, lipid peroxidation and excessive CYP2E1 expression and activity. The mice treated with different kaempferol dosages exhibited a significant decrease in the oxidative stress as well as lipid peroxidation, and increased anti-oxidative defense activity. The kaempferol treatment has significantly reduced the expression level and activity of hepatic CYP2E1, thus indicating that kaempferol could down regulate CYP2E1. These findings show the hepatoprotective properties of kaempferol against alcohol-induced liver injury by attenuating the activity and expression of CYP2E1 and by enhancing the protective role of anti-oxidative defense system.
- Subjects
ALCOHOLIC liver diseases; ANIMAL experimentation; ASPARTATE aminotransferase; BIOLOGICAL assay; BIOPHYSICS; BODY weight; GLUTATHIONE; RESEARCH methodology; LIPID peroxidation (Biology); MICE; OXIDOREDUCTASES; POLYMERASE chain reaction; RESEARCH funding; SUPEROXIDE dismutase; TOXICITY testing; WESTERN immunoblotting; MALONDIALDEHYDE; PLANT extracts; OXIDATIVE stress; ALANINE aminotransferase; DATA analysis software; DESCRIPTIVE statistics; IN vitro studies; ONE-way analysis of variance
- Publication
American Journal of Chinese Medicine, 2015, Vol 43, Issue 2, p241
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X15500160