We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Chemical Compositions, Anti-Inflammatory, Antiproliferative and Radical-Scavenging Activities of Actinidia callosa var. ephippioides.
- Authors
Liao, Jung-Chun; Deng, Jeng-Shyan; Chiu, Chuan-Sung; Huang, Shyh-Shyun; Hou, Wen-Chi; Lin, Wang-Ching; Huang, Guan-Jhong
- Abstract
Oxidative stress and inflammation are related to several chronic diseases including cancer. Actinidia callosa var. ephippioides (ACE) is a special folk medicinal plant from Taiwan. The aim of this study is to evaluate the antioxidant, anti-inflammatory, and antiproliferative activities of the methanol extract and fractions from the stem of ACE. Trolox Equivalent Antioxidant Capacity (TEAC), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, total phenolic content, flavonoid content, inhibition on nitric oxide (NO) productions by LPS-induced RAW264.7 cell, and on lung cancer cell proliferation were employed. Among all fractions, ethyl-acetate fraction (EA-ACE) showed higher TEAC, DPPH radical scavenging activities, polyphenol and flavonoid contents, respectively. EA-ACE also decreased the LPS-induced NO production and expressions of inducible nitric-oxide synthase (iNOS) in RAW264.7 cells. EA-ACE had the highest antiproliferative activity with an IC50 (The concentrations required for inhibition of 50% of cell viability) of 469.17 ± 3.59 μg/mL. Catechin also had good effects in the antioxidant and anti-inflammatory activities. Catechin might be an important bioactive compound in the stem of ACE. The above experimental data indicated that the stem of ACE is a potent antioxidant medicinal plant, and such efficacy may be mainly attributed to its polyphenolic compounds.
- Subjects
ANTI-inflammatory agents; THERAPEUTIC use of antioxidants; THERAPEUTIC use of plant extracts; ANALYSIS of variance; ANIMAL experimentation; CELL culture; CELL physiology; FLAVONOIDS; HIGH performance liquid chromatography; MACROPHAGES; MEDICINAL plants; CHINESE medicine; METHANOL; NITRIC oxide; POLYPHENOLS; REGRESSION analysis; RESEARCH funding; WESTERN immunoblotting; LIPOPOLYSACCHARIDES; FREE radical scavengers; DESCRIPTIVE statistics
- Publication
American Journal of Chinese Medicine, 2012, Vol 40, Issue 5, p1047
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X12500772