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- Title
Cook Your Own Ampholytes For Use In Isoelectric Focusing And Chromatofocusing.
- Authors
Kalaria, Tejas
- Abstract
Isoelectric focusing and chromatofocusing are techniques to separate amphoteric compounds based on their isoelectric points. The commonest used compounds to generate a pH gradient in these techniques are ampholytes. The prohibitively expensive cost of carrier ampholytes limits the application of these techniques in less advantaged parts of the world. A simple and inexpensive method is described in this paper to prepare a broad pH range (pH 3 to 11) ampholytes for use in isoelectric focusing or chromatofocusingin any routine laboratory. The method involves crosslinking polyethylene polyamine to create complex cross-linked polyaminesin the first step. Whereas, the second step involves reacting the cross-linked polyamines with an unsaturated carboxylic acid to produce ampholytes.
- Subjects
ISOELECTRIC focusing; ISOELECTRIC point; CARBOXYLIC acids; POLYAMINES; POLYETHYLENE
- Publication
National Journal of Integrated Research in Medicine, 2021, Vol 12, Issue 3, p105
- ISSN
2230-9969
- Publication type
Article