We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
厌氧微生物菌群XH-1对2,4,6-三氯苯酚的降解特性.
- Authors
孙百惠; 宋雪英; 严俊; 杨毅; 吕燕; 王晶晶; 宋玉芳; 李秀颖
- Abstract
Organic pollutant 2,4,6-trichlorophenol (2,4,6-TCP) is commonly found in anaerobic environments such as sediments and groundwater aquifers. To investigate the ability of the anaerobic consortium XH-1 to degrade 2,4,6-TCP, we established anaerobic incubations using 2,4,6-TCP as the substrate and inoculated the incubations with XH-1. Additional subcultures were established by amending with intermediate product 4-chlorophenol (4-CP) or phenol as the substrate. The transformation products of 2,4,6-TCP were analyzed and determined using high-performance liquid chromatography (HPLC). Microbial community structure and key microbial groups involved in the degradation of 2,4,6-TCP were analyzed based on 16S rRNA gene high-throughput sequencing. The results showed that the initial 122 μmol·L-1 2,4,6-TCP was completely transformed after a 80-day incubation at a rate of 0.15 μmol·d-1. 2,4-dichlorophenol (2,4-DCP), 4-CP and phenol were identified as the intermediate products. All intermediate products generated from 2,4,6-TCP transformation were completely degraded after being incubated for 325 days. The main microbial groups responsible for the reductive dechlorination of 2,4,6-TCP might be the organohalide respiring Dehalobacter and Dehalococcoides. The subsequent reductive dechlorination of 4-CP to phenol was likely driven by Dehalococcoides. The cooperation between the organohalide respiring bacteria, Syntrophorhabdus and methanogens (e.g. Methanosaeta and Methanofolis) was responsible for the complete degradation of 2,4,6-TCP.
- Subjects
HIGH performance liquid chromatography; NUCLEOTIDE sequencing; PHENOL; MICROBIAL communities; POLLUTANTS; TRICHLOROPHENOL
- Publication
Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao, 2022, Vol 33, Issue 12, p3395
- ISSN
1001-9332
- Publication type
Article
- DOI
10.13287/j.1001-9332.202212.030