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- Title
Comparative study of fungal stability between Metarhizium strains after successive subculture.
- Authors
Hussien, Rana H. M.; Ezzat, Said M.; El Sheikh, Ali A.; Taylor, James W. D.; Butt, Tariq M.
- Abstract
Background: Metarhizium species are considered one of the most outstanding powerful biological control agents that have been commercialized as biopesticides against various agricultural pests. Fungal stability with successive in vitro cultivation is a desirable trait for a large-scale production of fungal biopesticide. Main body: The new Egyptian strain Metarhizium anisopliae AUMC 3262 exhibited auspicious results when compared to Metarhizium brunneum ARSEF 4556 and M. brunneum V275 based on the variations of fungal characteristics, and essential quality control parameters (radial growth rate, conidial yield, viability, and virulence) after repeated in vitro subculturing. Changes in morphological characteristics were noted at both AUMC 3262 and ARSEF 4556. Following the 5th subculture, decreased conidial yield was noted, though radial growth remained stable, confirming that there is a non-positive correlation between conidial yield and radial growth rate for these species. In contrast, V275 showed a high morphological stability, conidial yield, and radial growth rate after repeated subculture. The three tested strains manifested high viability up to 100% and displayed the same pattern of Pr1 production. A slight variation was recorded in the median lethal time (LT50) values against the great wax moth, Galleria mellonella (L.), larvae between different subcultures of the tested Metarhizium strains. Conclusion: The new Egyptian strain AUMC 3262 showed a high stability with a slight difference in some parameters after the successive subculture compared to both ARSEF4556 and V275.
- Subjects
GREATER wax moth; BIOPESTICIDES; METARHIZIUM; BIOLOGICAL pest control agents; METARHIZIUM anisopliae; SUBCULTURES; AGRICULTURAL pests
- Publication
Egyptian Journal of Biological Pest Control, 2021, Vol 31, Issue 1, p1
- ISSN
1110-1768
- Publication type
Article
- DOI
10.1186/s41938-020-00348-4