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- Title
Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus.
- Authors
T. IKEGAMI; M. SAIJO; M. NIIKURA; M. E. MIRANDA; A. B. CALAOR; M. HERNANDEZ; D. L. MANALO; I. KURANE; Y. YOSHIKAWA
- Abstract
We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360739), and RΔ6 (aa 451551) and/or RΔ8 (aa 631739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.
- Subjects
IMMUNOGLOBULIN G; NUCLEOPROTEINS; EBOLA virus disease; AMINO acids
- Publication
Epidemiology & Infection, 2003, Vol 130, Issue 3, p533
- ISSN
0950-2688
- Publication type
Article
- DOI
10.1017/s0950268803008264