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- Title
Control of cleavage site selection during mRNA 3' end formation by a yeast hnRNP.
- Authors
Minvielle-Sebastia, Lionel; Beyer, Katrin; Krecic, Annette M.; Hector, Ron E.; Swanson, Maurice S.; Keller, Walter
- Abstract
Endonucleolytic cleavage of pre-mRNAs is the first step during eukaryotic mRNA 3′ end formation. It has been proposed that cleavage factors CF IA, CF IB and CF II are required for pre-mRNA 3′ end cleavage in yeast. CF IB is composed of a single polypeptide, Nab4p/Hrp1p, which is related to the A/B group of metazoan heterogeneous nuclear ribonucleoproteins (hnRNPs) that function as antagonistic regulators of 5′ splice site selection. Here, we provide evidence that Nab4p/Hrp1p is not required for pre-mRNA 3′ end endonucleolytic cleavage. We show that CF IA and CF II devoid of Nab4p/Hrp1p are sufficient to cleave a variety of RNA substrates but that cleavage occurs at multiple sites. Addition of Nab4p/Hrp1p prevents these alternative cleavages in a concentration-dependent manner, suggesting an essential and conserved role for some hnRNPs in pre-mRNA cleavage site selection.
- Subjects
SACCHAROMYCES cerevisiae; MESSENGER RNA; PEPTIDE hormones; NUCLEOPROTEINS; METAZOA
- Publication
EMBO Journal, 1998, Vol 17, Issue 24, p7454
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/17.24.7454