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- Title
Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
- Authors
Stocco, Elena; Barbon, Silvia; Zeni, Elena; Cassari, Leonardo; Zamuner, Annj; Gloria, Antonio; Russo, Teresa; Boscolo-Berto, Rafael; Sfriso, Maria Martina; Macchi, Veronica; De Caro, Raffaele; Dettin, Monica; Porzionato, Andrea
- Abstract
Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs). Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity. Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs. To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR. Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility. Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress. However, among +EAK groups, ChS+EAK showed a significantly higher maximum stress than that found for ChS+EAK-IKVAV and ChS+EAK-YIGSR. Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM. The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility. ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
- Subjects
TISSUE scaffolds; TISSUE engineering; CHITOSAN; SCANNING electron microscopy; PERIPHERAL nerve injuries; POLYCAPROLACTONE
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 20, p12059
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms232012059