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- Title
Characterization of CD4-Positive Lymphocytes in the Antiviral Response of Olive Flounder (Paralichthys oliveceus) to Nervous Necrosis Virus.
- Authors
Jung, Jae Wook; Chun, Jin Hong; Lee, Jung Seok; Kim, Si Won; Lee, Ae Rin; Kim, Jaesung; Lazarte, Jassy Mary S.; Kim, Young Rim; Kim, Hyoung Jun; Thompson, Kim D.; Jung, Tae Sung
- Abstract
The presence of CD4 T lymphocytes has been described for several teleost species, while many of the main T cell subsets have not been characterized at a cellular level, because of a lack of suitable tools for their identification, e.g., monoclonal antibodies (mAbs) against cell markers. We previously described the tissue distribution and immune response related to CD3ε and CD4-1 T cells in olive flounder (Paralichthys oliveceus) in response to a viral infection. In the present study, we successfully produce an mAb against CD4-2 T lymphocytes from olive flounder and confirmed its specificity using immuno-blotting, immunofluorescence staining, flow cytometry analysis and reverse transcription polymerase chain reaction (RT-PCR). Using these mAbs, we were able to demonstrate that the CD3ε T cell populations contain both types of CD4+ cells, with the majority of the CD4 T cell subpopulations being CD4-1+/CD4-2+ cells, determined using two-color flow cytometry analysis. We also examined the functional activity of the CD4-1 and CD4-2 cells in vivo in response to a viral infection, with the numbers of both types of CD4 T cells increasing significantly during the virus infection. Collectively, these findings suggest that the CD4 T lymphocytes in olive flounder are equivalent to the helper T cells in mammals in terms of their properties and function, and it is the CD4-2 T lymphocytes rather than the CD4-1 T cells that play an important role in the Th1 immune response against viral infections in olive flounder.
- Subjects
T helper cells; REVERSE transcriptase polymerase chain reaction; PARALICHTHYS; LYMPHOCYTES; T cells; MONOCLONAL antibodies; BOVINE viral diarrhea; TUMOR necrosis factors
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 11, p4180
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21114180