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- Title
Protective Actions of Anserine Under Diabetic Conditions.
- Authors
Peters, Verena; Calabrese, Vittorio; Forsberg, Elisabete; Volk, Nadine; Fleming, Thomas; Baelde, Hans; Weigand, Tim; Thiel, Christian; Trovato, Angela; Scuto, Maria; Modafferi, Sergio; Schmitt, Claus Peter
- Abstract
Background/Aims: In rodents, carnosine treatment improves diabetic nephropathy, whereas little is known about the role and function of anserine, the methylated form of carnosine. Methods: Antioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human renal tubular cells (HK-2) by RT-PCR and Western-Immunoblotting. In wildtype (WT) and diabetic mice (db/db), the effect of short-term anserine treatment on blood glucose, proteinuria and vascular permeability was measured. Results: Anserine has a higher antioxidant capacity compared to carnosine (p < 0.001). In tubular cells (HK-2) stressed with 25 mM glucose or 20–100 µM hydrogen peroxide, anserine but not carnosine, increased intracellular heat shock protein (Hsp70) mRNA and protein levels. In HK-2 cells stressed with glucose, co-incubation with anserine also increased hemeoxygenase (HO-1) protein and reduced total protein carbonylation, but had no effect on cellular sirtuin-1 and thioredoxin protein concentrations. Three intravenous anserine injections every 48 h in 12-week-old db/db mice, improved blood glucose by one fifth, vascular permeability by one third, and halved proteinuria (all p < 0.05). Conclusion: Anserine is a potent antioxidant and activates the intracellular Hsp70/HO-1 defense system under oxidative and glycative stress. Short-term anserine treatment in diabetic mice improves glucose homeostasis and nephropathy.
- Subjects
ANSERINE; DIABETIC nephropathies; CARNOSINE; RENAL tubular transport; HEAT shock proteins
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 9, p2751
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19092751