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- Title
Transgenic Plants as Low-Cost Platform for Chemotherapeutic Drugs Screening.
- Authors
Vergara, Daniele; de Domenico, Stefania; Maffia, Michele; Piro, Gabriella; Di Sansebastiano, Gian-Pietro
- Abstract
In this work we explored the possibility of using genetically modified Arabidopsis thaliana plants as a rapid and low-cost screening tool for evaluating human anticancer drugs action and efficacy. Here, four different inhibitors with a validated anticancer effect in humans and distinct mechanism of action were screened in the plant model for their ability to interfere with the cytoskeletal and endomembrane networks. We used plants expressing a green fluorescent protein (GFP) tagged microtubule-protein (TUA6-GFP), and three soluble GFPs differently sorted to reside in the endoplasmic reticulum (GFPKDEL) or to accumulate in the vacuole through a COPII dependent (AleuGFP) or independent (GFPChi) mechanism. Our results demonstrated that drugs tested alone or in combination differentially influenced the monitored cellular processes including cytoskeletal organization and endomembrane trafficking. In conclusion, we demonstrated that A. thaliana plants are sensitive to the action of human chemotherapeutics and can be used for preliminary screening of drugs efficacy. The cost-effective subcellular imaging in plant cell may contribute to better clarify drugs subcellular targets and their anticancer effects.
- Subjects
ARABIDOPSIS thaliana genetics; TRANSGENIC plants; CANCER chemotherapy; DRUG efficacy; DRUG use testing; GREEN fluorescent protein; GENE expression in plants; BIOCHEMICAL mechanism of action; THERAPEUTICS
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 1, p2174
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms16012174