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- Title
Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide.
- Authors
Yu-Bin Ji; Fang Dong; Lang Lang; Ling-Wen Zhang; Jing Miao; Zhen-Feng Liu; Li-Na Jin; Ying Hao
- Abstract
In this study, an experiment was designed to optimize the synthesis of seleno-Capparis spionosa L. polysaccharide (Se-CSPS) by response surface methodology. Three independent variables (reaction time, reaction temperature and ratio of Na2SeO3 to CSPS) were tested. Furthermore, the thermal stability, particle size, shape and cytotoxic activity of Se-CSPS in vitro were investigated. The optimum reaction conditions were obtained shown as follows: reaction time 7.5 h, reaction temperature 71 °C, and ratio of Na2SeO3 to CSPS 0.9 g/g. Under these conditions, the Se content in Se-CSPS reached 5.547 mg/g, which was close to the predicted value (5.518 mg/g) by the model. The thermal stability, particle size and shape of Se-CSPS were significantly different from those of CSPS. Additionally, a MTT assay indicated that the Se-CSPS could inhibit the proliferation of human gastric cancer SGC-7901 cells in a dose-dependent manner.
- Subjects
CAPPARIS spinosa; POLYSACCHARIDE synthesis; RESPONSE surfaces (Statistics); TEMPERATURE effect; CELL-mediated cytotoxicity; CANCER cells; ANTINEOPLASTIC agents; CHEMICAL reactions; EXPERIMENTS
- Publication
International Journal of Molecular Sciences, 2012, Vol 13, Issue 12, p17275
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms131217275