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- Title
Large-scale synthesis of tert-butyl ( 3R,5S)-6-chloro-3,5-dihydroxyhexanoate by a stereoselective carbonyl reductase with high substrate concentration and product yield.
- Authors
Liu, Zhi‐Qiang; Hu, Zhong‐Liang; Zhang, Xiao‐Jian; Tang, Xiao‐Ling; Cheng, Feng; Xue, Ya‐Ping; Wang, Ya‐Jun; Wu, Lin; Yao, Dan‐Kai; Zhou, Yi‐Teng; Zheng, Yu‐Guo
- Abstract
To biosynthesize the (3R,5S)-CDHH in an industrial scale, a newly synthesized stereoselective short chain carbonyl reductase (SCR) was successfully cloned and expressed in Escherichia coli. The fermentation of recombinant E. coli harboring SCR was carried out in 500 L and 5000 L fermenters, with biomass and specific activity of 9.7 g DCW/L, 15749.95 U/g DCW, and 10.97 g DCW/L, 19210.12 U/g DCW, respectively. The recombinant SCR was successfully applied for efficient production of (3R,5S)-CDHH. The scale-up synthesis of (3R,5S)-CDHH was performed in 5000 L bioreactor with 400 g/L of (S)-CHOH at 30°C, resulting in a space-time yield of 13.7 mM/h/g DCW, which was the highest ever reported. After isolation and purification, the yield and d.e. of (3R,5S)-CDHH reached 97.5% and 99.5%, respectively. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:612-620, 2017
- Subjects
CARBOXYLIC acids; CHEMICAL synthesis; FERMENTATION; CARBONYL reductase; ISOLATION of biotechnological microorganisms; PROTEIN fractionation; BIOCHEMICAL substrates; CLONING
- Publication
Biotechnology Progress, 2017, Vol 33, Issue 3, p612
- ISSN
8756-7938
- Publication type
Article
- DOI
10.1002/btpr.2460