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- Title
Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis.
- Authors
Şahin, Burcu; Öztürk, Sibel; Çalık, Pınar; Özdamar, Tunçer
- Abstract
Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 ( scoC) strain carrying pMK4:: pre( subC):: hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and (NH)HPO at two pre-determined specific growth rates, µ = 0.10 and 0.17 h. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, (NH)HPO, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ = 0.10, 0.17, and 0.25 h. At all the strategies applied, transition cultivation time from batch to fed-batch operation was t = 4 h. The highest rhGH concentration was obtained as C = 0.5 g L with semi-defined medium-based feeding strategy designed with µ = 0.25 h using feed substrate stock solution containing 200 g L glucose, 117 g L (NH)HPO, 100 g L peptone, and 5 mL L PTM1 at t = 22 h when the cell concentration reached to C = 8.29 g L. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg g and $$\bar{Y}_{X/S}$$ = 0.12 g g, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r- B. subtilis.
- Subjects
RECOMBINANT human somatotropin; BACILLUS subtilis biotechnology; HUMAN growth hormone; GLUCOSE; PHARMACEUTICAL industry
- Publication
Bioprocess & Biosystems Engineering, 2015, Vol 38, Issue 10, p1855
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-015-1426-3