We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Mutations of optineurin in amyotrophic lateral sclerosis.
- Authors
Maruyama, Hirofumi; Morino, Hiroyuki; Ito, Hidefumi; Izumi, Yuishin; Kato, Hidemasa; Watanabe, Yasuhito; Kinoshita, Yoshimi; Kamada, Masaki; Nodera, Hiroyuki; Suzuki, Hidenori; Komure, Osamu; Matsuura, Shinya; Kobatake, Keitaro; Morimoto, Nobutoshi; Abe, Koji; Suzuki, Naoki; Aoki, Masashi; Kawata, Akihiro; Hirai, Takeshi; Kato, Takeo
- Abstract
Amyotrophic lateral sclerosis (ALS) has its onset in middle age and is a progressive disorder characterized by degeneration of motor neurons of the primary motor cortex, brainstem and spinal cord. Most cases of ALS are sporadic, but about 10% are familial. Genes known to cause classic familial ALS (FALS) are superoxide dismutase 1 (SOD1), ANG encoding angiogenin, TARDP encoding transactive response (TAR) DNA-binding protein TDP-43 (ref. 4) and fused in sarcoma/translated in liposarcoma (FUS, also known as TLS). However, these genetic defects occur in only about 20–30% of cases of FALS, and most genes causing FALS are unknown. Here we show that there are mutations in the gene encoding optineurin (OPTN), earlier reported to be a causative gene of primary open-angle glaucoma (POAG), in patients with ALS. We found three types of mutation of OPTN: a homozygous deletion of exon 5, a homozygous Q398X nonsense mutation and a heterozygous E478G missense mutation within its ubiquitin-binding domain. Analysis of cell transfection showed that the nonsense and missense mutations of OPTN abolished the inhibition of activation of nuclear factor kappa B (NF-κB), and the E478G mutation revealed a cytoplasmic distribution different from that of the wild type or a POAG mutation. A case with the E478G mutation showed OPTN-immunoreactive cytoplasmic inclusions. Furthermore, TDP-43- or SOD1-positive inclusions of sporadic and SOD1 cases of ALS were also noticeably immunolabelled by anti-OPTN antibodies. Our findings strongly suggest that OPTN is involved in the pathogenesis of ALS. They also indicate that NF-κB inhibitors could be used to treat ALS and that transgenic mice bearing various mutations of OPTN will be relevant in developing new drugs for this disorder.
- Subjects
AMYOTROPHIC lateral sclerosis; MIDDLE age; MOTOR neurons; MOTOR cortex; BRAIN stem; SPINAL cord; GENES; DNA; LIPOSARCOMA
- Publication
Nature, 2010, Vol 465, Issue 7295, p223
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature08971