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- Title
Development of a Gene-Based Soybean-Origin Discrimination Method Using Allele-Specific Polymerase Chain Reaction.
- Authors
Jung, Kie-Chul; Kim, Bo-Young; Kim, Myoung-Jin; Kim, Nam-Kuk; Kang, Jihun; Kim, Yul-Ho; Park, Hyang-Mi; Jang, Han-Sub; Shin, Hee-Chang; Kim, Tae-Jip
- Abstract
A low soybean self-sufficiency rate in South Korea has caused a high import dependence and considerable price variation between domestic and foreign soybeans, causing the false labeling of foreign soybeans as domestic. Conventional soybean origin discrimination methods prevent a single-grain analysis and rely on the presence or absence of several compounds or concentration differences. This limits the origin discrimination of mixed samples, demonstrating the need for a method that analyzes individual grains. Therefore, we developed a method for origin discrimination using genetic analysis. The whole-genome sequencing data of the Williams 82 reference cultivar and 15 soybean varieties cultivated in South Korea were analyzed to identify the dense variation blocks (dVBs) with a high single-nucleotide polymorphism density. The PCR primers were prepared and validated for the insertion–deletion (InDel) sequences of the dVBs to discriminate each soybean variety. Our method effectively discriminated domestic and foreign soybean varieties, eliminating their false labeling.
- Subjects
SOUTH Korea; POLYMERASE chain reaction; SINGLE nucleotide polymorphisms; NUCLEOTIDE sequencing; GRAIN
- Publication
Foods, 2023, Vol 12, Issue 24, p4497
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods12244497