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- Title
Purification, Identification and Molecular Docking of Immunomodulatory Peptides from the Heads of Litopenaeus vannamei.
- Authors
Jiang, Weiwei; Ren, Keyu; Yang, Zhiyan; Fang, Zhou; Li, Yan; Xiang, Xi; Song, Yishan
- Abstract
In order to realize the high-value utilization of Litopenaeus vannamei (L. vannamei) heads, immunomodulatory peptides were prepared from the enzymatic hydrolysate of L. vannamei heads, and the action mechanism of immunomodulatory peptides was determined by molecular docking. The results showed that six proteases were used to hydrolyze L. vannamei head proteins, with the animal protease hydrolysate exhibiting the highest macrophage relative proliferation rate (MRPR). The enzymatic products were then sequentially purified by ultrafiltration, Sephadex G-15 gel chromatography, identified by liquid chromatography-mass spectrometry (LC-MS/MS), and finally selected for six immunomodulatory peptides (PSPFPYFT, SAGFPEGF, GPQGPPGH, QGF, PGMR, and WQR). These peptides maintained good immune activity under heat treatment, pH treatment, and in vitro gastrointestinal digestion. Molecular docking analysis indicated that these peptides showed great binding to both toll-like receptor 2 and 4 (TLR2 and TLR4/MD-2), leading to immunomodulation. The discarded L. vannamei heads in this article are considered to be promising food-borne immunomodulators that contribute to enhancing the immune function of the body.
- Subjects
WHITELEG shrimp; MOLECULAR docking; TOLL-like receptors; PEPTIDES; LIQUID chromatography-mass spectrometry; IMMUNOMODULATORS
- Publication
Foods, 2022, Vol 11, Issue 20, p3309
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods11203309