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- Title
Sandwich freezing device for rapid freezing of viruses, bacteria, yeast, cultured cells and animal and human tissues in electron microscopy.
- Authors
Yamaguchi, Masashi; Taguchi, Masaki; Uematsu, Katsuyuki; Takahashi-Nakaguchi, Azusa; Sato-Okamoto, Michiyo; Chibana, Hiroji
- Abstract
We have been using sandwich freezing of living yeast and bacteria followed by freeze-substitution for observing close-to-native ultrastructure of cells. Recently, sandwich freezing of glutaraldehyde-fixed cultured cells and human tissues have been found to give excellent preservation of ultrastructure of cells and tissues. These studies, however, have been conducted using a handmade sandwich freezing device and have been limited in a few laboratories. To spread the use of this method to other laboratories, we fabricated and commercialized a new sandwich freezing device. The new device is inexpensive, portable and sterilizable. It can be used to rapid-freeze viruses, bacteria, yeast, cultured cells and animal and human tissues to a depth of 0.2 mm if tissues are prefixed with glutaraldehyde. The commercial availability of this device will expand application of rapid freezing to wide range of biological materials.
- Subjects
HUMAN cell culture; ELECTRON microscopy; FREEZING; YEAST culture; BIOMATERIALS; CELL preservation
- Publication
Microscopy, 2021, Vol 70, Issue 2, p215
- ISSN
2050-5698
- Publication type
Article
- DOI
10.1093/jmicro/dfaa049