We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Construction of a Microsatellite-enriched Library of Rhododendron sirnsii Planch.
- Authors
Shuzhen WANG; Weibin JIN; Jun XIANG; Yongliang ZHENG; Yuanping FANG; Jianping GAN; Hua CHENG; Hang DU
- Abstract
In this study, an (AG) n microsatellite-enriched library of Rhododendron sirnsii Planch, was constructed with FIASCO (fat Isolation by AFLP sequences containing repeats) method. Among 356 positive colonies verified by colony PCR of the (AG)n microsateHite-enriched library, 233 colonies were selected for further sequencing. According to the results, 202 colonies containing microsateHites were obtained, accounting for 86.7%. Discarding sequences with misceHaneous peaks, overlapping peaks and weak signals, 39 sequences were screened finally for subsequent primer exploitation, including 22 perfect type SSR loci, 5 imperfect type SSR loci ad 12 compound type SSR loci. The FIASCO method is efficient and feasible for constructing microsatellite-enriched libraries of RRododendron spp., which laid a solid foundation for isolation of microsateHite loci and investigation of genetic diversity ad genetic structure of RRiododendron spp.
- Subjects
RHODODENDRONS; MICROSATELLITE repeats in plants; PLANT chromosomes; AZALEAS; PLANT genetics; GENETIC research
- Publication
Agricultural Biotechnology (2164-4993), 2015, Vol 4, Issue 5, p29
- ISSN
2164-4993
- Publication type
Article