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- Title
L-Arginine-Induced Regional Cerebral Blood Flow Increase is Abolished After Transient Focal Cerebral Ischemia in the Rat.
- Authors
Sporer, Bernd; Martens, K Helge; Koedel, Uwe; Haberl, Roman L
- Abstract
SummaryWe investigated the L-arginine-induced, regional cerebral blood flow (rCBF) enhancement after different durations of transient focal cerebral ischemia in the rat to determine if L-arginine increases rCBF after transient focal cerebral ischemia. Focal ischemia (5 minutes and 20 minutes) followed by 90 minutes of reperfusion was induced in a normotensive rat suture-model. Regional cerebral blood flow in both hemispheres was measured by laser-Doppler-flowmetry. Reactivity of rCBF to L-arginine (300 mg/kg) was measured 45 minutes after reperfusion, and hypercapnia 90 minutes after reperfusion. The effect of D-arginine and pretreatment with the nitric oxide (NO) synthase inhibitor Nω-nitro-L-arginine (L-NA) (10 mg/kg) was examined in additional groups. Hypercapnia and L-arginine increased rCBF in sham operated controls and on the nonischemic hemispheres. D-arginine did not. Twenty-minute long ischemia significantly reduced the response to L-arginine (control side: 115 ± 5.9%; ischemic side: 107 ± 6.1%, n = 7) and hypercapnia, 5 minutes of ischemia did not. Nω-nitro-L-arginine pretreatment partly restored the L-arginine-induced rCBF increase. Thus, rCBF increase caused by L-arginine in the reperfusion period was unaffected by 5 minutes of ischemia, but reduced by 20 minutes of ischemia. The restoration after pretreatment with L-NA may be caused by attenuated production of cytotoxic substances, e.g., NO and related compounds.Journal of Cerebral Blood Flow & Metabolism (1997) 17, 1074-1080; doi:10.1097/00004647-199710000-00009
- Publication
Journal of Cerebral Blood Flow & Metabolism, 1997, Vol 17, Issue 10, p1074
- ISSN
0271-678X
- Publication type
Article
- DOI
10.1097/00004647-199710000-00009