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- Title
A network of clinically and functionally relevant genes is involved in the reversion of the tumorigenic phenotype of MDA-MB-231 breast cancer cells after transfer of human chromosome 8.
- Authors
Seitz, Susanne; Frege, Renate; Jacobsen, Anja; Weimer, Jörg; Arnold, Wolfgang; Haefen, Clarissa von; Niederacher, Dieter; Schmutzler, Rita; Arnold, Norbert; Scherneck, Siegfried
- Abstract
Several investigations have supposed that tumor suppressor genes might be located on human chromosome 8. We used microcell-mediated transfer of chromosome 8 into MDA-MB-231 breast cancer cells and generated independent hybrids with strongly reduced tumorigenic potential. Loss of the transferred chromosome results in reappearance of the malignant phenotype. Expression analysis identified a set of 109 genes (CT8-ps) differentially expressed in microcell hybrids as compared to the tumorigenic MDA-MB-231 and rerevertant cells. Of these, 44.9%are differentially expressed in human breast tumors. The expression pattern of CT8-ps was associated with prognostic factors such as tumor size and grading as well as loss of heterozygosity at the short arm of chromosome 8. We identified CT8-ps networks suggesting that these genes act cooperatively to cause reversion of tumorigenicity in MDA-MB-231 cells. Our findings provide a conceptual basis and experimental system to identify and evaluate genes and gene networks involved in the development and/or progression of breast cancer.Oncogene (2005) 24, 869-879. doi:10.1038/sj.onc.1208260 Published online 6 December 2004
- Subjects
TUMOR suppressor genes; HUMAN chromosomes; CANCER cells; BREAST cancer; PHENOTYPES; CANCER genetics
- Publication
Oncogene, 2005, Vol 24, Issue 5, p869
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1208260