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- Title
An LOH and mutational investigation of the ST7 gene locus in human esophageal carcinoma.
- Authors
Wang, Suna; Mori, Yuriko; Sato, Fumiaki; Yin, Jing; Xu, Yan; Zou, Tong-Tong; Olaru, Andreea; Kimos, Martha C; Perry, Kellie; Selaru, Florin M; Deacu, Elena; Sun, Menghong; Shi, Ying-Chang; Shibata, David; Abraham, John M; Greenwald, Bruce D; Meltzer, Stephen J
- Abstract
Frequent loss of heterozygosity (LOH) on human chromosome 7q31 has been reported in numerous malignancies. Suppressor of tumorigenicity 7 (ST7) has been identified as a candidate tumor suppressor gene in this region. To identify whether 7q31 and genetic alterations of ST7 were involved in human esophageal carcinogenesis, we performed LOH mapping of a 5.4 cM region at 7q31-q35 in 43 primary esophageal carcinomas, as well as mutational analyses of the ST7 gene in tumors with LOH in this region. Of 43 tumors, 12 (28%) showed LOH at 7q31–q35. These included four (22%) of 18 squamous cell carcinomas and eight (32%) of 25 adenocarcinomas. The peak LOH locus was D7S480, lying 4.2 Mb telomeric to ST7 and showing LOH in eight of 37 informative tumors, or 22%. No mutations were found in the entire coding or flanking intronic regions of the ST7 gene among 12 tumors with 7q-LOH. In addition, quantitative RT–PCR analyses of ST7 mRNA expression levels in 11/13 normal-tumor pairs failed to show more than a 50% decrease in tumor ST7 mRNA relative to matched normal tissues. These data suggest that LOH at 7q31–q35 is involved in the origin or progression of at least a subset of esophageal carcinomas, but that ST7 is not the target gene of this somatic event.Oncogene (2003) 22, 467–470. doi:10.1038/sj.onc.1206125
- Subjects
HUMAN chromosomes; TUMOR suppressor genes
- Publication
Oncogene, 2003, Vol 22, Issue 3, p467
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1206125