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- Title
Actinomycin D Affects Thyrotropin-Releasing Hormone-Induced Heterogeneous Forms of Glycosylated Thyroid-Stimulating Hormone in Rat Anterior Pituitary.
- Authors
Mori, Masatomo; Michimata, Toshio; Yamaguchi, Masafumi; Yamada, Masanobu; Iriuchijima, Tokuji; Kobayashi, Setsuo
- Abstract
Actinomycin D and cycloheximide were used to clarify the tight relationship between protein synthesis and heterogeneous carbohydrate synthesis of rat pituitary thyroid-stimulating hormone (TSH) under thyrotropin-releasing hormone (TRH) stimulation in vitro. Rat anterior pituitaries were incubated with [3H]glucosamine in the presence of TRH, cycloheximide and actinomycin D at 37 °C for 3 and 6 h. The TSH fraction of pituitary homogenates was obtained using affinity chromatography coupled with anti-TSH globulin, and was analyzed by isoelectric focusing. Anterior pituitary in the presence of TRH showed heterogeneous components of [3H]glucosamine-labeled TSH with 6 different isoelectric points (components I-VI). Radioactivities of the components increased with the incubation period. Cycloheximide changed these heterogeneous components. Actinomycin D also caused a striking change in the heterogeneity of pituitary [3H]glucosamine-labeled TSH: components I and II decreased and components III and IV increased as compared to the control group after a 6-hour incubation. The present study implies that actinomycin D affects the TRH-induced heterogeneous components of pituitary TSH glycosylation. The data indicate that messenger RNA is essential for the normal processing of carbohydrate synthesis of pituitary TSH. Copyright © 1989 S. Karger AG, Basel
- Publication
Neuroendocrinology, 1989, Vol 50, Issue 5, p519
- ISSN
0028-3835
- Publication type
Article
- DOI
10.1159/000125274