We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Tüketime Sunulan Çiğ Sütlerde Escherichia coli O157:H7 Araştırılması.
- Authors
ÖNLEN GÜNERİ, Cansu; KIZILYILDIRIM, Suna
- Abstract
Objective: Escherichia coli (E.coli) O157:H7 is one of the important causes of morbidity and mortality in terms of human health. Most infections due to E. coli O157:H7 are foodborne. In this study, it was aimed to determine the prevalence of E. coli O157:H7 in raw milk offered for consumption in Mersin. Materials and Methods: Sixty raw milk samples sold in various markets and grocery stores in Mersin provience were examined. Each milk sample was taken into a sterile container and brought to the bacteriology laboratory in the cold chain. 25 ml of milk samples were taken and inoculated into 225 ml of Tryptic Soy Broth, which was prepared for enrichment. After enrichment, it was cultured on MacConkey or Eozin Metilen Blue (EMB) agar. After the suspicious colonies that grew at the end of the incubation were confirmed as E. coli by IMVIC test, they were inoculated on Sorbitol-Mac-Conkey (SMAC) agar to investigate E. coli O157:H7. Antibiotic susceptibility test was performed by Kirby Bauer disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines to investigate the isolated isolates in terms of antimicrobial resistance profiles. Results: E. coli O157:H7 was isolated in two (3.3%) of 60 raw milk samples analyzed. E. coli O157:H7 isolates were found to be resistant to ampicillin and chloramphenicol, whereas only one isolate was resistant to gentamicin and ciprofloxacin, while the other isolate was susceptible. In addition, both isolates were found to be sensitive to tetracycline, nalidixic acid and cefuroxime. Conclusion: It was concluded that raw milk and dairy products may be a source for E. coli O157:H7 and pose a risk to public health.
- Publication
SDU Journal of Health Science Institute / SDÜ Saglik Bilimleri Enstitüsü Dergisi, 2022, Vol 13, Issue 3, p441
- ISSN
2146-247X
- Publication type
Article
- DOI
10.22312/sdusbed.1202809