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- Title
Preparation, characterization, and cellular uptake of resveratrol-loaded trimethyl chitosan nanoparticles.
- Authors
Min, Jeong Bin; Kim, Eun Suh; Lee, Ji-Soo; Lee, Hyeon Gyu
- Abstract
The aim of the study was to encapsulate resveratrol (RV) in trimethyl chitosan (TMC) nanoparticles cross-linked with tripolyphosphate (TPP) and/or alginate to achieve controlled release and improved cellular uptake. TMC (degree of quaternization of 78%) was prepared by reacting purified chitosan with iodomethane. Three types of RV-loaded TMC nanoparticles were prepared: TMC-TPP (TP-NPs), TMC-alginate (TA-NPs), and TMC-alginate-TPP (TAP-NPs). TA-NPs and TAP-NPs showed lower particle size and encapsulation efficiency (EE), better distribution, and more sustained release than TP-NPs due to the high molecular weight and viscous property of alginate. Caco-2 cellular uptake of RV was improved by TMC nanoencapsulation, and TP-NPs showed the highest uptake due to its significantly higher EE. Compared with TAP-NPs, TA-NPs with higher positive surface charge showed higher cellular uptake. Moreover, Caco-2 cell growth-inhibiting activity of RV was significantly increased by TMC nanoencapsulation and TP-NPs showed the significantly highest activity with a good agreement with the permeability results.
- Publication
Food Science & Biotechnology, 2018, Vol 27, Issue 2, p441
- ISSN
1226-7708
- Publication type
Article
- DOI
10.1007/s10068-017-0272-2