We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Molecular Detection of the mcr Genes by Multiplex PCR.
- Authors
Liu, Jiayang; Zhang, Zhiyuan; Feng, Yuyang; Hu, Huimin; Yu, Yu; Qiu, Lihao; Liu, Hongtao; Guo, Zhimin; Huang, Jing; Du, Chongtao; Qiu, Jiazhang
- Abstract
Background: The emergence and prevalence of plasmid-mediated colistin-resistant bacterial strains in recent years have raised great concerns in clinical medicine. It is urgently needed to develop a cheaper, faster, simpler, sensitive, and specific molecular detection method to identify and monitor the dissemination of the transferable resistant determinants. Methods and Results: Herein, eight pairs of primers were designed to set up a multiplex PCR method for the rapid and efficient determination of reported mcr genes. This assay can give results within 85 min (35 min for amplification and 50 min for electrophoresis). We validated the feasibility of this assay by testing the presence of mcr genes in 60 colistin-resistant isolates. Conclusion: Our multiplex PCR technique exhibits remarkable advantages in the light of clear identification, efficiency of amplification, as well as the time consuming for detection, and thus could be useful for the surveillance and epidemiological research of plasmid-mediated colistin resistance, particularly for the under-resourced laboratories.
- Subjects
PLASMIDS; GENES; EPIDEMIOLOGICAL research; CLINICAL medicine
- Publication
Infection & Drug Resistance, 2020, Vol 13, p3463
- ISSN
1178-6973
- Publication type
Article
- DOI
10.2147/IDR.S256320