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- Title
STRUCTURE, MOLECULAR WEIGHT AND BIOACTIVITIES OF (1→3)-β-D-GLUCANS AND ITS SULFATED DERIVATIVES FROM FOUR KINDS OF LENTINUS EDODES.
- Authors
Surenjav, Unursaikhan; Zhang, Li-na; Xu, Xiao-juan; Zhang, Mei; Cheung, Peter Chi Keung; Zeng, Fan-bo
- Abstract
Lentinan samples, (1→3)-β-D-glucans containing 4.6–15.2 wt% proteins, coded as L-I1, L-I2, L-I3 and L-I4(L-I) were isolated from four kinds of Lentinus edodes. These glucans were treated with acetone to remove the protein in order to obtain free protein glucans coded as LNP-I1, LNP-I2, LNP-I3 and LNP-I4(LNP-I). The free-protein polysaccharides were sulfated to give derivatives (S-LNP-I) with degree of substitution (DS) from 0.4–0.8. The structural features and weight-average molecular weight (Mw) of the samples were investigated by using infrared spectroscopy, elemental analysis, 13C-NMR, size exclusion chromatography combined with laser light scattering (SEC-LLS) and viscometry. The effects of structure and conformation of the polysaccharides on antitumor activities were assayed in vivo (Sarcoma 180 solid tumors) and in vitro (Sarcoma 180, HL-60, MCF-7 and Vero tumors). The results indicated that the predominant species of the samples L-I and LNP-I in 0.2 mol/L NaCl aqueous solution existed as triple-helical chains with high rigidity and in dimethyl sulfoxide (DMSO) as single-flexible chains. Interestingly, the antitumor activities of LNP-I are lower than those of the native glucans (L-I), whereas their sulfated derivatives have higher inhibition ratio against Sarcoma 180 than LNP-I. The results reveal that the binding of protein, sulfated modification and the triple helix conformation are important factors in the enhancement of the antitumor activities of polysaccharides on the whole.
- Subjects
GLUCANS; POLYSACCHARIDES; MOLECULAR weights; PROTEINS; SARCOMA; DIMETHYL sulfoxide
- Publication
Chinese Journal of Polymer Science (World Scientific Publishing Company), 2005, Vol 23, Issue 3, p327
- ISSN
0256-7679
- Publication type
Article
- DOI
10.1142/S0256767905000461