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- Title
Rapid Isolation/Selection of Best Yeast Culture and Its Metabolic Control for the Biotransformation of Benzaldehyde to 1-Hydroxy-1- phenyl-2-propanone.
- Authors
Akhtar, Naeem; Javed, Muhammad Mohsin; Zahoor, Sana; Ikram-ul-Haq; Babar, Masroor Elahi
- Abstract
Present work describes the rapid isolation and selection of thirteen yeast culture having high resistance to acetaldehyde and benzaldehyde, directly added to the medium at isolation stage. All isolates had 100% similarity with type strain Saccharomyces cerevisiae during molecular characterization. S. cerevisiae APL-2 showed better kinetic parameters and ultimately lead to better production of 1-hydroxy-1-phenyl-2-propanone. This fermentation involved two phases, first phase to attain the suitable level of cell density while second phase is the biotransformation phase. Effect of temperature (15-30°C) was studied during second phase (at the time of acetaldehyde and benzaldehyde dozing). The maximum level of biotransformation was achieved when temperature was maintained at 15-18°C. Addition of benzyl alcohol also enhanced the biotransformation level.
- Subjects
YEAST research; SACCHAROMYCES cerevisiae; BIOTRANSFORMATION (Metabolism); BENZALDEHYDE; ACETALDEHYDE; ORGANIC compounds
- Publication
Pakistan Journal of Zoology, 2014, Vol 46, Issue 3, p783
- ISSN
0030-9923
- Publication type
Article