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- Title
CDK-5-Mediated Neurofilament Phosphorylation in SHSY5Y Human Neuroblastoma Cells.
- Authors
Sharma, Monica; Sharma, Pushkar; Pant, Harish C.
- Abstract
Cyclin-dependent kinase-5 (CDK-5) has been shown to play important roles in neuronal development and neurogenesis. In vitro studies indicate a role of CDK-5 in phosphorylation of neurofilaments (NFs). In this study, we have chosen the human neuroblastoma cell line SHSY5Y as a model system to study the in vivo phosphorylation of NF proteins by CDK-5. Upon differentiation of SHSY5Y cells with retinoic acid, we found that the phosphorylation of high molecular mass (NF-H) and medium molecular mass (NF-M) NFs increased, whereas the CDK-5 protein level and kinase activity were unaffected. The role of CDK-5 in the phosphorylation of cytoskeletal proteins was studied by using antisense oligonucleotides (ONs) to inhibit the expression of the CDK-5 gene. We found that inhibition of CDK-5 levels by antisense ON treatment resulted in a decrease in phosphorylation of NF-H that correlated with a decline in neurite outgrowth. These results demonstrate that CDK-5 is a major proline-directed kinase phosphorylating the human NF-H tail domain.
- Subjects
PHOSPHORYLATION; NEUROBLASTOMA; CYCLIN-dependent kinases; CYTOSKELETAL proteins
- Publication
Journal of Neurochemistry, 1999, Vol 73, Issue 1, p79
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1046/j.1471-4159.1999.0730079.x