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- Title
Contribution of environmental factors in the formation of biofilms by Alicyclobacillus acidoterrestris on surfaces of the orange juice industry.
- Authors
Dutra, Tatiane Viana; Biral do Prado, Daniela; dos Anjos, Márcia Maria; Machinski Junior, Miguel; Graton Mikcha, Jane Martha; Alves de Abreu Filho, Benício
- Abstract
The objective of this study was to evaluate the effect of the initial microbial load, temperature and contact time on the biofilm formation of Alicyclobacillus acidoterrestris on stainless steel and natural food-grade rubber using orange juice as culture medium. The low initial load of A. acidoterrestris (2 log CFU/mL) led to biofilm formation on the stainless steel surface after 48 h of contact at 28 °C and after 24 h at 45 °C, and on natural food-grade rubber surface after 48 h of contact at both temperatures. The high initial microbial load (5 log CFU/mL) led to biofilm formation on stainless steel after 4 h of contact at 28 °C and 45 °C, while biofilm was formed on natural food-grade rubber after 8 h of contact at 28 °C and 4 h at 45 °C. The microbial load also affected the presence of spores in biofilm, which was observed on both surfaces only at high initial loads of A. acidoterrestris.
- Publication
Ciência Rural, 2020, Vol 50, Issue 3, p1
- ISSN
0103-8478
- Publication type
Article
- DOI
10.1590/0103-8478cr20190790