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- Title
Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy.
- Authors
Zhang, Li; Yilmaz, Ahmet; Chian, Ri-Cheng; Son, Weon-Young; Zhang, Xiao; Kong, Dewen; Dahan, Michael; Holzer, Hannanel; Tan, Seang; Ao, Asangla
- Abstract
Purpose: To evaluate preimplantation genetic diagnosis (PGD) efficiency in thawed human embryos vitrified without biopsy. Methods: In this retrospective clinical study, 21 PGD cycles were carried out using fresh and vitrified-thawed embryos collected from 21 patients. Results: One hundred and ninety-nine embryos from patients with aneuploidy, single gene defects, or chromosomal translocations were vitrified at the cleavage stages; 93.5% of the embryos survived the vitrification procedure. Conclusive FISH results were obtained in 98.4% of the fresh and 99% of the frozen-thawed embryos screened for aneuploidy or chromosomal translocations. Conclusive PCR test results were obtained in 100% of the fresh and 93.9% of the frozen-thawed embryos screened for single gene defects. The overall clinical pregnancy rate per cycle was 57.1%. To date, 13 healthy children have been born. Conclusion: Reliable genetic diagnosis can be performed in thawed human embryos vitrified without biopsy. However, further research is required to support this conclusion.
- Subjects
PREIMPLANTATION genetic diagnosis; FROZEN human embryos; ANEUPLOIDY; FERTILIZATION in vitro; CHROMOSOMAL translocation; OBSTETRICS; BIOPSY
- Publication
Journal of Assisted Reproduction & Genetics, 2011, Vol 28, Issue 7, p597
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-011-9556-2