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- Title
Isolation and characterisation of carbapenem-resistant Xanthomonas citri pv. mangiferaeindicae-like strain gir from the faecal material of giraffes.
- Authors
McFarland, Renee; Anacker, Melissa; Snippes Vagnone, Paula M.; Dowd, Scot E.; Henken, Susan; McLaughlin, Richard William
- Abstract
The purpose of this study was to determine if giraffes (Giraffa camelopardalis) living in captivity at the Jacksonville Zoo and Gardens, Jacksonville, FL were colonised with carbapenem-resistant bacteria and, if found, to identify underlying genetic mechanisms contributing to a carbapenem-resistant phenotype. Faecal samples from seven giraffes were examined for carbapenem-resistant bacteria. Only one isolate (a Xanthomondaceae) was found to be carbapenem-resistant by antibiotic susceptibility testing. This isolate was selected for additional characterization, including whole genome sequencing (WGS). Based on average nucleotide identity, the bacterium was identified as Xanthomonas citri pv. mangiferaeindicae-like strain gir. Phenotypic carbapenemase tests and PCR for the most common carbapenemase genes produced negative results, suggesting that carbapenem resistance was mediated by another mechanism. Resistance gene profile analysis of WGS results confirmed these results. Among identified resistance genes, a chromosomal class A beta-lactamase with 71% identity to the penP beta-lactamase gene from Xanthomonas citri ssp. citri was identified, which could contribute to a carbapenem-resistant phenotype.
- Subjects
JACKSONVILLE (Fla.); XANTHOMONAS campestris; FECES; GIRAFFES; CARBAPENEMS; NUCLEOTIDE sequencing; ANTIBIOTICS testing
- Publication
Antonie van Leeuwenhoek, 2020, Vol 113, Issue 1, p137
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-019-01323-2