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- Title
Acetylsalicylic acid as antifungal in Eremothecium and other yeasts.
- Authors
N. Leeuw; C. Swart; D. Ncango; C. Pohl; O. Sebolai; C. Strauss; P. Botes; P. van Wyk; S. Nigam; J. Kock
- Abstract
Abstract Interesting distribution patterns of acetylsalicylic acid (ASA, aspirin) sensitive 3-hydroxy (OH) oxylipins were previously reported in some representatives of the yeast genus Eremothecium—an important group of plant pathogens. Using immunofluorescence microscopy and 3-OH oxylipin specific antibodies in this study, we were able to map the presence of these compounds also in other Eremothecium species. In Eremothecium cymbalariae, these oxylipins were found to cover mostly the spiky tips of narrowly triangular ascospores while in Eremothecium gossypii, oxylipins covered the whole spindle-shaped ascospore with terminal appendages. The presence of these oxylipins was confirmed by chemical analysis. When ASA, a 3-OH oxylipin inhibitor, was added to these yeasts in increasing concentrations, the sexual stage was found to be the most sensitive. Our results suggest that 3-OH oxylipins, produced by mitochondria through incomplete β-oxidation, are associated with the development of the sexual stages in both yeasts. Strikingly, preliminary studies on yeast growth suggest that yeasts, characterized by mainly an aerobic respiration rather than a fermentative pathway, are more sensitive to ASA than yeasts characterized by both pathways. These data further support the role of mitochondria in sexual as well as asexual reproduction of yeasts and its role to serve as a target for ASA antifungal action.
- Subjects
ASPIRIN; LEAVENING agents; EDIBLE fungi; EREMOTHECIUM
- Publication
Antonie van Leeuwenhoek, 2007, Vol 91, Issue 4, p393
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-006-9124-4