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- Title
<sup>1</sup>H HR-MAS spectroscopy for quantitative measurement of choline concentration in amniotic fluid as a marker of fetal lung maturity: Inter- and intraobserver reproducibility study.
- Authors
Joe, Bonnie N.; Vahidi, Kiarash; Zektzer, Andrew; Chen, Mei-Hsiu; Clifton, Matthew S.; Butler, Thomas; Keshari, Kayvan; Kurhanewicz, John; Coakley, Fergus; Swanson, Mark G.
- Abstract
Purpose To determine the intra- and interobserver reproducibility of human amniotic fluid metabolite concentration measurements (including potential markers of fetal lung maturity) detectable by MR spectroscopy. Materials and Methods 1H high-resolution magic angle spinning (HR-MAS) spectroscopy was performed at 11.7T on 23 third-trimester amniotic fluid samples. Samples were analyzed quantitatively using 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSP) as a reference. Four observers independently quantified eight metabolite regions (TSP, lactate doublet and quartet, alanine, citrate, creatinine, choline, and glucose) twice from anonymized, randomized spectra using a semiautomated software program. Results Excellent inter- and intraobserver reproducibility was found for all metabolites. Intraclass correlation as a measure of interobserver agreement for the four readers ranged from 0.654 to 0.995. A high correlation of 0.973 was seen for choline in particular, a major component of surfactant. Pearson correlation as a measure of intraobserver reproducibility ranged from 0.478 to 0.999. Conclusion Quantification of choline and other metabolite concentrations in amniotic fluid by high-resolution MR spectroscopy can be performed with high inter- and intraobserver reproducibility. Demonstration of reproducible metabolite concentration measurements is a critical first step in the search for biomarkers of fetal lung maturity. J. Magn. Reson. Imaging 2008;28:1540-1545. © 2008 Wiley-Liss, Inc.
- Publication
Journal of Magnetic Resonance Imaging, 2008, Vol 28, Issue 6, p1540
- ISSN
1053-1807
- Publication type
Article
- DOI
10.1002/jmri.21592