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- Title
Development of Nitroreductase‐Activatable Fluoroquinolone Prodrugs Exhibiting Attenuated Magnesium Ion Binding.
- Authors
Zhao, Zhenxiang; Yadav, Anuj K.; Weng, Yourong; Chan, Jefferson
- Abstract
Fluoroquinolones such as levofloxacin and ciprofloxacin are potent antibiotics prescribed to treat various bacterial infections. Recent studies have also identified prominent examples as promising anti‐cancer agents. However, significant off‐target effects observed in human patients have precluded their wide‐spread clinical use. For instance, upon systemic administration, overt toxicity toward eukaryotic organelles (i. e., mitochondria) can result to the destruction of healthy tissue. Moreover, fluoroquinolones feature a β‐ketoacid motif which binds to and strips magnesium ions from cartilage, leading to rupture of the Achilles tendon. In this study, we have masked the β‐ketoacid of levofloxacin and ciprofloxacin (as well as two quinolones) with a nitroreductase (NTR)‐responsive warhead appended through a new self‐immolative prodrug linker. The resulting β‐ketoester functional group was found to be remarkably resistant toward spontaneous hydrolysis, esterase activity, as well as other ester‐cleaving enzymatic systems. Further testing also reveal no cross‐reactivity against commonly encountered biologically relevant analytes (e. g., ROS and thiols). Importantly, we demonstrate that upon esterification, the binding toward magnesium ions was attenuated based on an in vitro fluorescent competition assay with the unmodified parent drug.
- Subjects
MAGNESIUM ions; FLUOROQUINOLONES; CIPROFLOXACIN; PRODRUGS; ACHILLES tendon rupture; BINDING site assay; ANTINEOPLASTIC agents
- Publication
Helvetica Chimica Acta, 2023, Vol 106, Issue 10, p1
- ISSN
0018-019X
- Publication type
Article
- DOI
10.1002/hlca.202300100