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- Title
[sup 18] F-FDG and [sup 18] F-FET uptake in experimental soft tissue infection.
- Authors
Kaim, Achim H.; Weber, Bruno; Kurrer, Michael O.; Westera, Gerrit; Schweitzer, Alain; Gottschalk, Jochen; von Schulthess, Gustav K.; Buck, Alfred
- Abstract
The aim of this study was to compare the uptake of [sup 18]F-fluoroethyl-L-tyrosine ([sup 18]F-FET) with that of [sup 18]F-fluorodeoxyglucose ([sup 18]F-FDG) in activated inflammatory white blood cells. Unilateral thigh muscle abscesses were induced in 11 rats by intramuscular inoculation of 0.1 ml of a bacterial suspension (S. aureus, 1.2x10[sub 9] CFU/ml). Four animals were intraperitoneally injected with 130-180 MBq [sup 18]F-FDG, four with 140-170 MBq [sup 18]F-FET and three with a mixture of 140-170 MBq [sup 18]F-FET and 1.8 MBq [sup 14]C-deoxyglucose. Autoradiography (10 µm slice thickness) of the abscess and the contralateral muscle was performed and detailed spatial correlation of autoradiography and histopathology (haematoxylin-eosin staining) was obtained. Regions of interest were placed on the abscess wall and the grey values (digitised image intensities) measured were converted to kBq/cc per kBq injected activity per gram (SUV). Areas with increased [sup 18]F-FDG uptake corresponded to cellular inflammatory infiltrates mainly consisting of granulocytes. The SUV was calculated to be 4.08±0.65 (mean±SD). The uptake of [sup 18]F-FET in activated white blood cells was not increased: the SUV of the abscess wall, at 0.74±0.14, was even below that of contralateral muscle. The low uptake of [sup 18]F-FET in non-neoplastic inflammatory cells promises a higher specificity for the detection of tumour cells than is achieved with [sup 18]F-FDG, since the immunological host response will not be labelled and inflammation can be excluded.
- Subjects
AMINO acids; AUTORADIOGRAPHY
- Publication
European Journal of Nuclear Medicine & Molecular Imaging, 2002, Vol 29, Issue 5, p648
- ISSN
1619-7070
- Publication type
Article
- DOI
10.1007/s00259-002-0780-y