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- Title
"Antibacterial effect and possible mechanism of action of 1,3,4-oxadiazole in Staphylococcus aureus".
- Authors
Formagio, Maíra Dante; Silva, João Vitor de Oliveira; Silva, Alex Fiori; Campanerut-Sá, Paula Aline Zanetti; Urbano, Alexandre; Bonfim-Mendonça, Patrícia de Souza; Capoci, Isis Regina Grenier; Cotica, Érika Seki Kioshima; Mikcha, Jane Martha Graton
- Abstract
Staphylococcus aureus is one of the main etiological agents causing foodborne diseases, and the development of new antibacterial agents is urgent. This study evaluated the antibacterial activity and the possible mechanism of action of the 1,3,4-oxadiazole LMM6 against S. aureus. The minimum inhibitory concentration (MIC) of LMM6 ranged from 1.95 to 7.81 µg ml−1. The time-kill assay showed that 48-h treatment at 1× to 8× MIC reduced S. aureus by 4 log colony forming unit (CFU), indicating a bacteriostatic effect. Regarding the possible mechanism of action of LMM6, there was accumulation of reactive oxygen species (ROS) and an increase in the absorption of crystal violet (∼50%) by the cells treated with LMM6 at 1× and 2× MIC for 6–12 h. In addition, there was increased propidium iodide uptake (∼84%) after exposure to LMM6 for 12 h at 2× MIC. After 48 h of treatment, 100% of bacteria had been injured. Scanning electron microscopy observations demonstrated that LMM6-treated cells were smaller compared with the untreated group. LMM6 exhibited bacteriostatic activity and its mechanism of action involves increase of intracellular ROS and disturbance of the cell membrane, which can be considered a key target for controlling the growth of S. aureus.
- Subjects
FOODBORNE diseases; REACTIVE oxygen species; GENTIAN violet; SCANNING electron microscopy; ANTIBACTERIAL agents; BOVINE mastitis; CELL membranes
- Publication
Letters in Applied Microbiology, 2024, Vol 77, Issue 1, p1
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1093/lambio/ovad138