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- Title
Hydrogen Peroxide Increases the Iron-Catalysed Induction of DNA Single-Strand Breaks by Asbestos.
- Authors
Aust, A. E.; Chao, C.-C.; Lund, L. G.
- Abstract
The effect of H2O2 on iron-catalysed induction of single-strand breaks in ϕX174 RFI DNA by crocidolite or amosite was determined in the presence of chelators with or without ascorbate. Crocidolite or amosite (1 mg ml−1) did not induce any DNA single-strand breaks (SSB) in ϕX174 RFI DNA. However, when ascorbate (1 mM) was added, both forms of asbestos induced SSB and addition of 25 μM H2O2 increased the SSB by 25–100%. Crocidolite or amosite incubated with 1 mM nitrilotriacetate (NTA) induced DNA SSB, and again the addition of H2O2 increased the DNA SSB by at least 25% for both forms of asbestos. When crocidolite or amosite was incubated with ϕX174 DNA, citrate (1 mM) and ascorbate (1 mM), there was 32% DNA damage induced by crocidolite and 52% by amosite. Addition of H2O2 increased the induction of SSB by 40% or more for both forms of asbestos. These results suggest that the simultaneous exposure of lung epithelial cells or mesothelial cells in vivo to iron associated with asbestos and H2O2 released from activated macrophages or cigarette smoke may be more damaging than exposure to asbestos alone.
- Publication
Annals of Occupational Hygiene, 1994, Vol 38, p595
- ISSN
0003-4878
- Publication type
Article