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- Title
Comparison of Three Methods for Extraction of Bacterial DNA from Bacteriemia Blood Samples.
- Authors
ZHAO Xiao-li; LI Xiao-xia; LIU De-hua; REN Bao-jun; SHAO Jian-chun; HU Da-chun
- Abstract
Objective To find an effective method for extracting bacterial DNA from bacteriemia blood samples. Methods Bacterial DNA from bacteriemia blood samples was extracted by three methods including alkali /heat lysis,lysozyme digestion and commercial DNA extraction kit. PCR with the specific primers for common pathogenic bacteria was used to amplify the extracted DNA. Result The PCR results showed that the methods of alkali/heat lysis was better than other two methods for extracting DNA from both Gram-negative and Gram-positive bacteria. Conclusion Alkali/heat lysis method is the most simple and effective method for extracting DNA from clinical common pathogenic bacteria.
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2014, Vol 35, Issue 4, p117
- ISSN
2095-610X
- Publication type
Article